Biocontrol of Soil Borne Pathogen of Potato Tuber Caused by Rhizoctonia solani through Biosurfactant based Bacillus strain

Black scurf disease is a major problem for cultivation of potato worldwide that managed by synthetic agrochemicals, are increasing environmental pollution as well residue in farm produce also. Therefore, now day use bio-agents managing black attractive feature and need the hour. A promising bio surfactant OD02 isolate, was isolated from oil contaminated soil, utilizing its secondary metabolite after preliminary biosurfactant screening tests. Furthermore, this strain exhibited positive test with haemolytic activity, emulsification index, spreading test, molecular characterization 16S rRNA universal primers revealed belongs to Bacillus group, further chemically characterized using TLC, exhibiting reddish pink colour lipopeptide demonstrated antagonistic activity against Rhizoctonia solani under vitro dual culture (45±0.30%) at 3% concentration food poisoning method. subtilis HussainT-AMU very effective phytopathogen R. can be more explored future diseases management strategies.